Blood groups

The most important blood groups in transfusion are the ABO blood group system and the group D of the Rh system.

Blood groups or antigens are determined by either sugars or proteins on the surface of the red blood cell. The ABO system has A and B antigens and the Rh system has many antigens but D is the most important.

There are 35 blood group systems. This means a person who is A Rh D positive may also be K positive (Kell system), M and N positive (MNS system) and Fya positive but Fyb negative (Duffy system) and so on.   

The ABO blood group systemarrow

If you have blood group A then you have got the A antigen on your red cells.

Blood group B means you have the B antigen, while group O has neither, and group AB has both A and B antigens.

Group O has neither A nor B antigens on the surface of their red cells so red cells of this group can be given safely to any other group. This is why group O donors are known as 'universal red cell donors. Group O makes both anti-A and anti-B antibodies.

The ABO system has associated anti-A and anti-B antibodies, antibodies being the body’s natural defence against foreign antigens. These antibodies are found in the plasma and are unusual in that they are ‘naturally occuring’ so they don’t need to be stimulated – such as by having a transfusion or during pregnancy. This means that blood from someone with blood group A contains anti-B antibodies.

Similarly, blood from someone with blood group B contains anti-A antibodies and those with group O contain both anti-A and anti-B whilst those with group AB have neither anti-A nor anti-B. 

Giving someone blood from the wrong ABO group could be life-threatening.

For instance, the anti-A antibodies in a recipient with group B blood group will attack the group A cells if this transfused to them. This is why group A blood must never be given to a group B person.

The Rh systemarrow

Another important blood group system in transfusion is the Rh system.

There are 5 main Rhesus (Rh) antigens on red cells – C, c, D, E, e.

The most important of these is the Rh D group. The D antigen on the red cell is what gives you the positive (+) or the negative (-) after the letter A, B, AB or O.

It is the most immunogenic and most likely to cause a transfusion reaction in the recipient.

To prevent transfusion reactions and production of alloantibody, Rh D negative girls and women of child bearing potential should not be transfused with Rh D positive red cells except in an emergency.

Alloantibody produced by transfusion of Rh D positive blood to Rh D negative women (or produced by exposure during sensitisation events in pregnancy such as ante partum haemorrhage /bleeding in pregnancy/miscarriage when an Rh D negative mother is carrying an Rh D positive baby) can cause Haemolytic Disease of the Fetus and Newborn (HDFN).

HDFN occurs when either during a previous transfusion or a previous pregnancy as described above, alloantibody in mother’s blood crosses the placenta, binding to the fetal red cells. This breaks down the fetal red blood cells causing anaemia referred to as haemolytic anaemia. The three most important red cell antibodies in clinical practice are RhD (anti D), Rhc (anti c) and Kell (anti Kell).

83% of our donors have the D antigen on their red blood cells and are D positive.

The remaining 17% lack the D antigen and are D negative. (Source – NHS Blood and Transplant; Active Donor Base 2013/14).

As well as D the Rh blood group system also has CE genes.

CE produces four combinations; ce, Ce, cE or CE

The most frequently occurring phenotypes of Rh CE and Rh D are Dce, cde, DCe, dCe, DcE, dcE, DCE and dCE.

Sometimes we will communicate specifically with groups of donors who have specific phenotypes such as those who have the Dce or the ‘Ro’ subtype or phenotype. While Ro blood is rare amongst White European people, it is much more common amongst Black African and African Caribbean people.

The Ro phenotype is over 10 times as common in individuals from Black backgrounds compared to individuals from White backgrounds and is very common in patients with sickle cell disease.

Ro blood is needed for patients who have Ro blood group. This allows a closer match for these patients and reduces the development of alloantibodies.

Alloimmunnisation is exacerbated by differences in blood group distribution between patients with Sickle Cell Disease (SCD) and the predominantly white European blood donor population.

A significant proportion of SCD and Thalasseamia patients have Ro phenotype cDe which is rare in donors of European origin. These groups of patients require on going blood transfusions. It is important that we group and type donors specifically (extended phenotype) for patients with this particular blood group as they require regular blood transfusions. Transfusion of red cells for these patients should be matched for ABO, D, C, E c, e and Kell.

This fact that Ro donors are much more commonly found in the Black and Minority Ethnic (BAME) population is one of the reasons why we are asking many more BAME donors to donate blood.

Ro blood is needed for patients who also have Ro blood. Often, these patients are those who require ongoing blood transfusions - many of our sickle cell patients.

The Distribution of Blood Groups

The chart below shows the distribution of blood groups amongst our donors. It's important that we collect enough of the right type of blood in the right quantities to meet the needs of hospitals and patients across England.

ABO Blood Group% of donor population with this group% of blood issued to hospitals
A + 30% 30%
A - 8% 8%
B + 8% 8%
B - 2% 2%
O + 36% 36%
O - 12% 13%
AB + 3% 2%
AB - 1% 1%

Data accurate at 31 March 2016.  
(Percentage figures have been rounded to the nearest whole number)